recombinant mouse mature region gdf9 (R&D Systems)
Structured Review

Recombinant Mouse Mature Region Gdf9, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant mouse mature region gdf9/product/R&D Systems
Average 93 stars, based on 27 article reviews
Images
1) Product Images from "Bone Morphogenetic Protein 15 in the Pro-Mature Complex Form Enhances Bovine Oocyte Developmental Competence"
Article Title: Bone Morphogenetic Protein 15 in the Pro-Mature Complex Form Enhances Bovine Oocyte Developmental Competence
Journal: PLoS ONE
doi: 10.1371/journal.pone.0103563
Figure Legend Snippet: Granulosa cell tritiated thymidine incorporation following exposure to mature GDF9, mature BMP15 or pro-mature BMP15 at 100 ng/ml. Bars represent mean ± SEM and different lowercase letters indicate a statistically significant difference (P<0.05). Data were derived from 5 independent replicates.
Techniques Used: Derivative Assay
Figure Legend Snippet: Effect of supplementing COC during IVM with different forms of BMP15 (mature BMP15 and pro-mature BMP15), mature GDF9 at 100 ng/ml dose, with and without FSH on oocyte developmental competence.
Techniques Used: Control
Figure Legend Snippet: Effect of supplementing COC during IVM with different forms of BMP15 (mature BMP15 and pro-mature BMP15), mature GDF9 at 100 ng/ml dose, with and without FSH on blastocyst quality.
Techniques Used: Control
Figure Legend Snippet: Spent IVM medium were analysed for glucose and lactate levels post 23(mature BMP15 and pro-mature BMP15) or mature GDF9 at 100 ng/ml, in the absence or presence of FSH. A. Glucose uptake. B. Lactate production. Bars represent the mean ± SEM. Data were derived from 12 independent replicates for each treatment.
Techniques Used: Derivative Assay
Figure Legend Snippet: Effect of treatment of intact COCs with different forms of BMP15 (mature BMP15 and pro-mature BMP15) or mature GDF9 at 100 ng/ml, +/− FSH on intra-oocytemetabolic activity. A. Autofluorescence of NAD(P)H. B. Autofluorescence of FAD. C. REDOX ratio (FAD/NAD(P)H). D. GSH levels. Bars represent the mean ± SEM. Data were derived from 4 independent replicates for autofluorescence on intra-oocyte NAD(P)H, FAD, and REDOX ratio and 3 independent replicates for GSH levels. Columns with different superscripts are significantly different (P<0.05); a,b minus FSH, x–y plus FSH.
Techniques Used: Activity Assay, Derivative Assay
Figure Legend Snippet: Representative micrographs of intra-oocyte NAD(P)H (A, C) and FAD (B, D) autofluorescence and GSH fluorescence (E, F), after treatment with different forms of BMP15 (mature BMP15 and pro-mature BMP15) or mature GDF9 at 100 ng/ml, in the absence (A, B, E) or presence (C, D, F) of FSH.
Techniques Used: Fluorescence